College of Chemistry Course Guide

BIOE 168L - Practical Light Microscopy (3 Units)

(Taken from the UC Berkeley Course Guide)

Course Overview

Summary

This laboratory course is designed for students interested in obtaining practical hands-on training in optical imaging and instrumentation. Using a combination of lenses, cameras, and data acquisition equipment, students will construct simple light microscopes that introduce basic concepts and limitations important in biomedical optical imaging. Topics include compound microscopes, Kohler illumination, Rayleigh two-point resolution, image contrast including dark-field and fluorescence microscopy, and specialized techniques such as fluorescence recovery after photobleaching (FRAP). Intended for students in both engineering and the sciences, this course will emphasize applied aspects of optical imaging and provide a base of practical skill and reference material that students can leverage in their own research or in industry.

Prerequisites

None

Workload

Time Commitment

2 hours of lecture and 3 hours of laboratory per week.




UC Berkeley Course Guide